LAP LAMBERT Academic Publishing 2014. New. Metabolic engineering targets for the production of high-value compounds. Citrus peel is produced ~15000000 t per year worldwide causing environmental problems. Its main constituent is D-galacturonic acid and can be converted to useful chemicals using genetically engineered strains. Engineered Aspergillus niger were used in solid and submerged state fermentation to convert orange peel to L-galactonate in a consolidate process. The gaaB coding for L-galactonate dehydrogenase was deleted gaaB and ii the gaaB was deleted and the gaaA coding for D-galacturonate reductase was overexpressed gaaB-gaaA. gaaB was able to convert up to 87 % of D-galacturonic acid to L-galactonate. Also in this work the eukaryotic D-Glucuronic acid pathway was studied. A decarboxylase that converts 3-keto-L-gulonate to L-xylulose remains poorly characterized and the gene is not known. A coupled enzyme assay was used to detect its activity. In the assay ammonium sulfate precipitates from bovine liver extract were coupled with engineered L-gulonate-3-dehydrogenase and L-xylulose reductase. A 3-keto-L-gulonate decarboxylase activity could not be detected. LAP LAMBERT Academic Publishing unknown
Ссылка продавца : BD5-9783659580345 ISBN : 3659580341 9783659580345
�ditions universitaires europ�ennes 2018. New. L�objectif global de cette �tude est de mener les observations ph�nologiques en conditions exp�rimentales des graines de Caloncoba wilwitschii en p�pini�re dans les conditions �cologiques de Kikwit et ses environs. Les objectifs op�rationnels consistent � : Etablir un dispositif exp�rimental des graines Caloncoba wilwitschii; D�terminer le taux de germination de l�esp�ce concern�e; Produire les plantules de l�esp�ce �tudi�e; Observer la croissance en hauteur et en diam�tre; Suivre l��volution de l�apparition des organes v�g�tatifs a�riens. Notre travail a �t� consist� de mener les essais des graines et des boutures de Myrianthus arboreus en p�pini�re dans les conditions exp�rimentales dans de la r�gion de Kikwit et ses environs. Les observations ont �t� men�es pendant 5 mois. Les r�sultats obtenus sont encourageants et ouvrent la voie aux scientifiques d�utiliser le mat�riel au reboisement et boisement des terres d�grad�es afin de permettre de r�pondre � la politique REDD . Nous sugg�rons aux autres chercheurs d�approfondir les connaissances sur d�autres param�tres que nous n�avons pas abord� durant notre travail . �ditions universitaires europ�ennes unknown
Ссылка продавца : BD5-9786138409274 ISBN : 6138409272 9786138409274
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